Figure 6. Dominant-Negative Rab5 and Rab7 Do Not Block Acquisition of lamp-1 by the C. jejuni–Containing Vacuole.

Cos-1 cells were transfected with plasmids expressing either Rab5-GFP, Rab7-GFP, or the dominant negative mutants Rab5^S34N-GFP or Rab7^N125I-GFP. Twenty-four hours after transfection, cells were infected with C. jejuni or S. typhimurium invA (invasin) for 15 min followed by a 1 h gentamicin chase. Cells were fixed, and lamp-1 acquisition was assessed after processing for immunofluorescence using anti-C. jejuni or anti-S. typhiumurium and lamp-1 antibodies. Results are the means and standard deviation of three independent experiments. For each experiment, at least 100 vacuoles were counted for colocalization with lamp-1.