Table 1.

Acetaldehyde production from pyruvate catalyzed by P. furiosus POR

Conditions	Acetaldehyde produced, μmol	Relative activity, %
Complete assay*	7.72	100
−pyruvate	0.028	0.4
−TPP	7.62	99
−CoA	0.056	0.7
−Fd	7.40	96
−POR	0.066	0.9
+air†	0.070	0.9
+desulfocoenzyme A‡	8.03	104

Acetaldehyde production was measured at 80°C under standard assay conditions as described. 

^*The 2 ml assay mixture contained 10 mM pyruvate, 0.1 mM TPP, 1.0 mM CoA, 5 μM P. furiosus Fd, and 92 μg P. furiosus POR in 50 mM CAPS (3-[cyclohexylamino]-l-propanesulfonic acid), pH 10.2. 

^†The reaction was carried out under aerobic conditions. 

^‡Complete assay without CoA and with desulfocoenzyme A (1.0 mM).