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. Author manuscript; available in PMC: 2008 Sep 7.
Published in final edited form as: Cell. 2008 Mar 7;132(5):875–886. doi: 10.1016/j.cell.2008.02.019

Figure 5. miR-17~92 regulates early B cell survival.

Figure 5

(A) Representative flow cytometry plots of bone marrow cells from mice reconstituted with wild-type or miR-17~92-deficient fetal liver cells. (B) Percentage of pro-B and pre-B cells in reconstituted mice. The median (red bars) and the p-values are indicated. (C) Representative flow cytometry plots showing relative proportion of fraction A–C pro-B cells in the bone marrow of reconstituted mice. (D) Histogram overlays of Annexin-V staining of pro-B (left panel) and pre-B cells (right panel), showing increased apoptosis of pro-B cells in the bone marrow of mice reconstituted with miR-17~92-deficient fetal liver cells. The analysis was performed on the bone marrow of mice reconstituted with wild-type (red line) or miR-17~92-deficient (blue line) fetal livers. (E) qPCR analysis of miR-17~92 and miR-106b~25 expression during B cell development. Pro-, pre-, immature and mature B cells were sorted and their RNA assayed for miR-20a and miR-93 expression. Data are normalized to sno-142 expression.