Table 4.
BstEII and HaeIII fragment lengths (base pairs) for 30 isolates with new patterns by PRA-hsp65.
| Speciesa | PRA-hsp65 | N | Fragment BstEII | Length (bp) HaeIII |
| M. arupense | NP17 | 5 | 320-115 | 145-75-60 |
| M. avium | NP10 | 1 | 320-115 | 140-90-60 |
| M. cosmeticum | NP6 | 1 | 320-115 | 150-95-80 |
| M. fortuitum | NP12 | 1 | 235-120-85 | 140-120-100-55 |
| M. fortuitum | NP19 | 1 | 235-120-100 | 145-140-100-55 |
| M. gordonae | NP1 | 11 | 235-120-100 | 130-110-95 |
| M. gordonae | NP3 | 1 | 320-130 | 130-60 |
| M. gordonae | NP13 | 1 | 235-120-85 | 130-90 |
| M. gordonae | NP22 | 1 | 235-130-85 | 160-90-60 |
| M. mageritense | NP5 | 1 | 240-130-85 | 145-100-50 |
| M. nonchromogenicum | NP4 | 1 | 235-120-85 | 145-80-60 |
| M. sherrisii | NP14 | 3 | 235-120-85 | 145-130 |
| M. terrae | NP11 | 2 | 235-210 | 140-115-70 |
a Species identification based on sequencing of hsp65 gene. Bold indicates sequences submitted to GenBank and patterns included in the updated PRA-hsp65 algorithm (see Figures 1, 2 and 3). GenBank accession numbers: NP1, EF601222; NP11, EF601223; NP14, AY365190 [23]; NP17, DQ168662 [18].
All isolates with new PRA-hsp65 profiles were cultured from sputum, with the following exceptions: NP1: urine (2), feces, liver biopsy and unknown (one each); NP17: unknown (2).