Table 5.
Phenotypic characteristics of isolates demonstrating previously unreported PRA-hsp65 patterns.
| Species | PRA hsp65 | 25°C | 37°C | 45°C | pg | TCH | nit | Tween | NaCl | Aryl3 | Aryl15 | ag | pic | β-gal | LJ | HA | PNB | INH | RF | EMB | CIP | OFL |
| M. arupense | NP17 | 3 | 3 | 0 | N | 2–3 | 0 | 1 | 0 | 0–1 | 0–2 | nd | 0 | 0–1 | nd | 3 | 2–3 | 3 | 0 | 0 | 0–1 | 3 |
| M. avium | NP10 | 3 | 3 | 0 | N | 3 | 3 | 1 | 1 | 2 | 3 | 3 | 0 | 0 | 3 | 3 | 1 | 1 | 1 | 0 | 0 | 0 |
| M. cosmeticum | NP6 | 3 | 3 | 2 | N | 3 | 0 | 1 | 0 | 1 | 2 | 1 | 2 | 1 | nd | 2 | 3 | 2 | 3 | 2 | 1 | 2 |
| M. fortuitum | NP12 | 3 | 3 | 0 | S | 3 | 3 | 1 | 3 | 1 | 3 | 2 | 1 | 0 | 3 | 3 | 3 | 3 | 3 | 3 | 0 | 3 |
| M. fortuitum | NP19 | 3 | 3 | 0 | N | 3 | 3 | 0 | 3 | 3 | 3 | 3 | 3 | 1 | 3 | 3 | 3 | 3 | 3 | 3 | 0 | 2 |
| M. gordonae | NP1 | 2–3 | 3 | 0 | S | 3 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 3 | 1–3 | 3 | 0–3 | 0–3 | 0–1 | 0–1 | 1–3 |
| M. gordonae | NP3 | 3 | 1 | 0 | S | 3 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 |
| M. gordonae | NP13 | 1 | 2 | 0 | P | 3 | 1 | 0 | nd | 0 | 1 | 0 | 0 | 0 | 3 | 3 | 3 | 0 | 1 | 1 | 2 | 1 |
| M. gordonae | NP22 | 3 | 3 | 3 | S | 3 | 3 | 2 | 0 | 0 | 0 | nd | 0 | 0 | 3 | 0 | 3 | 1 | 3 | 3 | 0 | nd |
| M. mageritense | NP5 | 3 | 3 | 0 | N | 3 | 2 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 3 | 3 | 3 | 0 | 3 | 0 | 0 | 0 |
| M. nonchromogenicum | NP4 | 2 | 3 | 2 | N | 2 | 1 | 1 | 0 | 0 | 0 | nd | 1 | 1 | nd | 2 | 2 | 2 | 2 | 0 | 0 | 0 |
| M. sherrisii | NP14 | 1 | 2 | 0 | S | 1–2 | 0 | 0 | 1–2 | 0 | 0 | 1 | 0 | 1 | 2 | 1–2 | 1–2 | 1 | 1 | 2 | 2 | 1–2 |
| M. terrae | NP11 | 2 | 3 | 0 | N/S | 2–3 | 0–3 | 1, 2 | 1 | 0 | 0–1 | 0 | 0 | 0–1 | 3 | 3 | 1, 2 | 3 | 0 | 0 | 0, 1 | 3 |
Phenotypes: 24°C, 36°C, 45°C: growth at temperature shown; pg: pigmentation (N, nonchromogen; P, photochromogen; S, scotochromogen); TCH: growth on thiophene-2-carboxylic acid hydrazide; nit: nitrate reduction; Tween: hydrolysis of Tween 80; NaCl: growth on 5% NaCl; Aryl 3. Aryl 15: arylsulfatase activity after 3 and 15 days of growth, respectively; ag: growth on nutrient agar; pic: growth on picric acid; β-gal: β-galactosidase activity; LJ: growth on Löwenstein-Jensen media; HA: growth on hydroxylamine 500 μg/ml; PNB: growth on p-nitrobenzoic acid; INH: isoniazid; RF: rifampicin; EMB: ethambutol; CIP: ciprofloxacin; OFL: ofloxacin. Responses are graded 0 (negative, no growth, no activity expressed) to 3 (positive, heavy growth, strong activity expressed); nd, not done. For patterns with multiple isolates, the result shown represents the most common phenotype(s) or the range of phenotypes observed.