Table 5.
Proteins associated with glioma vessel formation established from enzymatic digested by trypsin peptide masses that are exclusively present in the "glioma vessels" group.
| Measured mass MH+ (Da) | Calculated mass MH+ (Da) | ppm | p-value Intensity H against S | Intensity H > S | Glioma vessels (H) | Normal vessels (S) | Glioma tissue(TH) | Normal tissue (TS) |
| SwissProtTM accession code Q9UKI2, name: Cdc42 effector protein 3 (Binder of Rho GTPases 2) | ||||||||
| 1229.6644 | 1229.66365 | 0.61 | 0.00411 | yes | 6 | 0 | 0 | 0 |
| 1656.8022 | 1656.80295 | 0.45 | 0.13120 | yes | 5 | 1& | 0 | 0 |
| 1620.7609 | 1620.76073 | 0.10 | 0.25509 | yes | 4 | 0 | 0 | 0 |
| 1772.8870 | 1772.88871 | 0.96 | 0.80459 | no | 3 | 3 | 1 | 1 |
| SwissProtTM accession code, name: Fibronectin precursor (FN) (Cold-insoluble globulin) (CIG) | ||||||||
| 1807.9063 | 1807.90469 | 0.89 | 0.00089 | yes | 8 | 0 | 0 | 0 |
| 1355.6954 | 1355.69536 | 0.03 | 0.00125 | yes | 7 | 0 | 0 | 0 |
| 1625.8539 | 1625.85196 | 1.19 | 0.00232 | yes | 5 | 0 | 0 | 0 |
| 1349.6848* | 1349.68481 | 0.01 | 0.00345 | yes | 7 | 0 | 0 | 0 |
| 1926.0516 | 1926.04832 | 1.70 | 0.00503 | yes | 8 | 1& | 0 | 0 |
| 1629.8728* | 1629.87067 | 1.31 | 0.00873 | yes | 5 | 0 | 0 | 0 |
| 2470.3200 | 2470.31874 | 0.51 | 0.01233 | yes | 7 | 1 | 1 | 0 |
| 1078.5537 | 1078.55275 | 0.88 | 0.01835 | no | 0 | 3 | 1 | 1 |
| 1818.9739 | 1818.97078 | 1.72 | 0.02049 | yes | 5 | 0 | 0 | 0 |
| 2524.3649 | 2524.36568 | 0.31 | 0.02049 | yes | 5 | 0 | 0 | 0 |
| 1401.6649* | 1401.66580 | 0.64 | 0.02170 | yes | 6 | 0 | 0 | 0 |
| 1341.6739 | 1341.67570 | 1.34 | 0.02663 | yes | 5 | 1 | 3 | 0 |
| 1110.5413 | 1110.54257 | 1.14 | 0.06243 | yes | 4 | 0 | 2 | 0 |
| 1593.8126 | 1593.81186 | 0.46 | 0.06243 | yes | 6 | 1 | 1 | 0 |
| 1292.6740 | 1292.67324 | 0.59 | 0.06373 | yes | 5 | 1 | 0 | 1 |
| 1297.7117 | 1297.71102 | 0.52 | 0.09473 | yes | 3 | 0 | 1 | 1 |
| 1591.8091 | 1591.80744 | 1.04 | 0.12668 | yes | 4 | 0 | 2 | 0 |
| 1235.5266 | 1235.52620 | 0.32 | 0.13120 | yes | 3 | 1 | 0 | 1 |
| 1431.7507 | 1431.74915 | 1.08 | 0.18754 | yes | 5 | 2 | 0 | 0 |
| 2139.9619 | 2139.96514 | 1.51 | 0.45543 | yes | 5 | 1 | 6 | 0 |
| 858.4301 | 858.43157 | 1.71 | 0.95436 | no | 2 | 1 | 2 | 0 |
| 1291.7265 | 1291.72560 | 0.70 | 1.00000 | yes | 3 | 3 | 1 | 0 |
| SwissProtTM accession code Q99653, name: Calcium-binding protein p22 (Calcium-binding protein CHP) (Calcineurin homologous protein) | ||||||||
| 960.5588 | 960.55850 | 0.31 | 0.02082 | yes | 6 | 0 | 4 | 0 |
| 1138.5646 | 1138.56262 | 1.74 | 0.00959 | yes | 5 | 0 | 0 | 1 |
| 1508.7103 | 1508.70876 | 1.02 | 0.05363 | yes | 5 | 0 | 0 | 0 |
| SwissProtTM accession code P35579, name: Myosin-9 (Myosin heavy chain, non-muscle IIa) | ||||||||
| 1869.9677* | 1869.96645 | 0.67 | 0.00170 | yes | 6 | 0 | 0 | 0 |
| 1155.6643 | 1155.66325 | 0.91 | 0.00310 | yes | 5 | 0 | 0 | 0 |
| 1949.9955 | 1949.99268 | 1.45 | 0.01285 | yes | 8 | 0 | 0 | 0 |
| 2493.1740* | 2493.17392 | 0.03 | 0.04085 | yes | 6 | 0 | 0 | 0 |
| 2472.1699 | 2472.17356 | 1.48 | 0.05363 | yes | 5 | 0 | 0 | 0 |
| 1393.7099# | 1393.71102 | 0.80 | 0.07335 | no | 1 | 4 | 3 | 7 |
| 1946.0059 | 1946.00765 | 0.90 | 0.33559 | yes | 7 | 4 | 6 | 0 |
| 924.4946 | 924.49377 | 0.90 | 0.60387 | yes | 3 | 2 | 3 | 0 |
| 1309.6023† | 1309.60188 | 0.32 | 0.61715 | yes | 4 | 3 | 1 | 0 |
| 1193.6166* | 1193.61606 | 0.45 | 0.75200 | yes | 3 | 2 | 4 | 2 |
| 1310.7038$ | 1310.70626 | 1.88 | 1.00000 | yes | 1 | 0 | 7 | 0 |
The listed proteins are found by a MASCOT™ search with 2 or more hits between measured and calculated tryptic peptide masses with an error tolerance of 2 ppm. Only proteins are listed that had at least one peptide mass with a Wilcoxon-Mann-Whitney p-value < 0.01 comparing the peak intensities of glioma vessels with normal vessels.
* WARP-LC MS-MS MALDI-TOF glioma tissue and vessels (see Additional file 1).
# SwissProtTM accession code: P40925, name: Malate dehydrogenase, cytoplasmic, identified by WARP-LC MS-MS on glioma tissue and vessels and normal tissue and vessels (see Additional file 1).
† SwissProtTM accession code: P08670, name: Vimentin, sequence: NLQEAEEWYK, MALDI TOF MS-MS by hand on the “glioma tissue and vessels” LC-MALDI plate using the BiotoolsTM 3.0 (build 1.68) software.
$ SwissProtTM accession code: P62805, name: Histone H4, identified by WARP-LC MS-MS on glioma tissue and vessels and normal tissue and vessels (see Additional file 1).
& outlier sample S10.