Figure 4.
Intercellular lipids and extracellular lipid were detected by Oil Red O staining with the cryosections from adult mice (A) and senescent mice (B). Arrows show the positive staining (red). The images were photographed at 100x magnification. Periodic acid Schiff (PAS) staining on paraffin embedded slides from adult mice (C) and senescent mice (D) were used for detection of carbohydrate content (Glycogen). Images were photographed at 40x magnification. In the senescent mice, oil red o staining revealed increased intracellular lipid accumulation while periodic acid schiff staining showed a concomitant decrease in glycogen stores.