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. Author manuscript; available in PMC: 2008 Apr 20.
Published in final edited form as: Cell. 2008 Mar 21;132(6):958–970. doi: 10.1016/j.cell.2008.01.018

Figure 6. Role of H3K4me2 in FoxA1 recruitment to the chomatin.

Figure 6

A-C) Effect of KDM1 over-expression on H3K4 methylation (A), FoxA1 recruitment (B) and H3K9 methylation (C). H3K4me2 and H3K9me2 levels as well as FoxA1 recruitment were determined in control or KDM1 over-expressing cells by ChIP-qPCR. Box plots were generated from data obtained for 16 sites. Results from one representative experiment are presented with the statistical analyses of the difference between control and KDM1 over-expressing cells, **: p≤0.01. D) Western blots showing KDM1, FoxA1 and Calnexin (Control) levels in MCF7 cells transfected with an empty control plasmid or a plasmid coding for KDM1. E) Specific examples of genes regulated by E2, DHT or by both hormones. One gene specifically regulated by E2 in MCF7 cells (MCF7-only), by DHT in LNCaP cells (LNCaP only) and by both hormones in MCF7 and LNCaP cells respectively (both) are shown. E2 and DHT regulated genes were identified using expression array analyses performed in MCF7 and LNCaP cells, respectively. Significantly regulated genes were determined using a t-test and a p-value cut-off of 5×10-3. ERα, AR and FoxA1 binding sites from ChIP-chip are indicated together with the occurrence of histone modifications derived from ChIP-qPCR at these sites. Enrichment for the various factors is presented by green and red blocks in LNCaP and MCF7 cells, respectively. White blocks indicate the absence of enrichment for the ChIPed factors or a decrease of more than two-fold for histone marks in MCF7 cells following KDM1 over-expression. A 4 kb wide view of the probe signals obtained by ChIP-chip for FoxA1, ERα and AR at the analyzed binding sites is also shown. Complete probe signal across the 3 genes selected is presented in Fig.S21.