Table 2. Fold change of the expression of selected genes in tumor vs normal liver tissues of mice.

ΔC_T values were calculate using mRNA levels of β-actin as the normalization standard. The values shown are the mean ± S.D. of RB positive normal (n = 3) and tumor (n =4) liver samples and RB-negative normal (n = 3) and tumor (n = 8) liver samples. Fold-change was calculated by raising 2 to the power of ΔC_T^normal minus ΔC_T^tumor; a value <1 indicates less mRNA in tumors than in normal tissues, whereas a value >1 indicates higher mRNA levels in tumors than in normal tissues. P-values were calculated using Student’s t test and are considered significant if ≤ 0.05.

RB Positive Tumors					RB Negative Tumors
Gene	ΔCT Normal	ΔCT Tumor	Fold Change	p value	ΔCT Normal	ΔCT Tumor	Fold Change	p value
Ahr	8.2 ± 0.1	10.0 ± 0.6	0.3 ± 0.2	0.004	8.8 ± 0.2	9.5 ± 0.2	0.6 ± 0.1	<0.001
Cdkn1a (p21)	12.5 ± 0.8	10.2 ± 1.4	4.9 ± 0.7	0.01	8.8 ± 3.1	8.3 ± 0.5	1.4 ± 1.0	N.S.
Cdkn1b (p27)	9.0 ± 0.3	9.7 ± 0.7	0.6 ± 0.3	N.S.	10.2 ± 1.0	9.4 ± 0.2	1.7 ± 0.3	N.S.
Cdkn2b (p15)	19.7 ± 3.6	19.7 ± 3.2	1.0 ± 2.0	N.S.	18.8 ± 2.0	19.1 ± 1.4	0.8 ± 0.8	N.S.
Cdkn2c (p18)	11.6 ± 0.2	9.5 ± 1.0	4.3 ± 0.4	0.02	12.2 ± 1.5	9.5 ± 0.6	6.5 ± 0.5	0.001
Cdkn2d (p19)	10.3 ± 0.3	11.4 ± 0.4	0.5 ± 0.2	0.01	12.4 ± 1.8	11.1 ± 0.4	2.5 ± 0.6	N.S.
Cyp1a1	14.2 ± 0.8	20.1 ± 4.7	0.0 ± 2.0	N.S.	20.3 ± 1.4	19.0 ± 2.3	2.5 ± 0.9	N.S.
Dnmt1	11.8 ± 0.2	12.2 ± 0.7	0.8 ± 0.3	N.S.	11.2 ± 0.5	11.0 ± 0.4	1.1 ± 0.2	N.S.
Hdac1	8.0 ± 0.4	8.4 ± 0.3	0.8 ± 0.2	N.S.	8.6 ± 0.4	8.2 ± 0.3	1.3 ± 0.2	N.S.
Nqo1	8.8 ± 0.7	9.4 ± 2.1	0.7 ± 0.9	N.S.	10.4 ± 1.4	10.6 ± 1.6	0.9 ± 0.7	N.S.
Pai1	12.6 ± 0.4	10.9 ± 1.0	3.2 ± 0.4	<0.001	12.1 ± 2.2	10.6 ± 1.4	2.8 ± 0.8	N.S.
Tgfb1	8.2 ± 0.1	9.4 ± 0.2	0.4 ± 0.1	<0.001	8.8 ± 0.5	8.4 ± 0.3	1.3 ± 0.2	N.S.
Tgfb2	14.4 ± 1.4	11.0 ± 0.5	10.6 ± 0.6	0.006	14.5 ± 1.1	11.4 ± 0.8	8.6 ± 0.4	<0.001
Tgfb3	18.6 ± 1.2	15.1 ± 1.0	11.3 ± 0.6	0.008	16.3 ± 1.3	15.3 ± 1.2	2.0 ± 0.6	N.S.
Trp53	6.7 ± 0.2	6.5 ± 0.6	1.1 ± 0.3	N.S.	6.3 ± 0.6	6.1 ± 0.3	1.1 ± 0.2	N.S.