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. 2008 Apr 30;3(4):e2041. doi: 10.1371/journal.pone.0002041

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Synthesis scheme for the immuno-affinity matrix for BoNT-enrichment. Protein A-agarose beads are coupled to affinity-purified anti-BoNT antibodies. Disuccinimidyl suberate (DSS) is used to crosslink the FC domain of the antibodies to protein A. Non-crosslinked antibodies are removed by stringent washing. (B) Immobilized BoNT/A cleaves a quenched FRET pair, provided in form of a fluorogenic peptide substrate, thereby releasing the fluorophore from the quencher, which restores fluorescence.