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. 2008 Apr 30;3(4):e2041. doi: 10.1371/journal.pone.0002041

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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BoNT/A detection in (A) 10% fetal bovine serum (FBS) to various forms and sources of the toxin; ^aMetabiologics (Madison, WI) and ^bList Biological Laboratories (Campbell, CA); (B) in undiluted human serum, 50% carrot juice (with binding buffer), gelatin phosphate diluent (GPD), reconstituted powdered milk (fat free) and fresh whole milk (2% fat). Pre-act. = toxin preactivated with 5 mM DTT; all reactions with SNAPtide were carried out with 1.25 mM DTT.