. Author manuscript; available in PMC: 2009 Apr 1.

Published in final edited form as: Bioorg Chem. 2008 Feb 11;36(2):96–104. doi: 10.1016/j.bioorg.2007.12.003

Table 2.

Spectral properties of Kaede-like chromophores.

Chrommophore	Absorption max, nm (extinction coefficient, mM⁻¹cm⁻¹) in neutral solutions^[a]					pKa (water)	Absorption max, nm (extinction coefficient, mM⁻¹cm⁻¹) in basic solutions^[b]					Emission max, nm
	Water	EtOH	ⁱPrOH	DMF	DMSO		Water	EtOH	ⁱPrOH	DMF	DMSO	(QY)^[c]
HYG	430	443	444	446	448	7.7	490	502	514	557	565	582
	(41)	(40)	(41)	(40)	(37)		(53)	(79)	(80)	(77)	(80)	(0.005)
WYG	460	462	460	460	465	8.1	494	509	532	580	595	615
	(7)	(15)	(16)	(14)	(14)		(21)	(24)	(27)	(27)	(27)	(0.017)
FWG	455	456	460	456	462	11.6	498^[d]	508	526	556	563	625
	(11)	(23)	(25)	(20)	(19)		(13)	(23)	(26)	(22)	(24)	(0.016)
YWG	462	466	468	464	468	8.3	496	509	533	567	577	610
	(8)	(30)	(26)	(23)	(22)		(27)	(34)	(40)	(38)	(44)	(0.017)
NYG	424	433	433	431	435	7.6	510	531	548	564	543	642
	(27)	(19)	(18)	(21)	(18)		(35)	(36)	(30)	(34)	(25)	(0.005)
FYG	425	437	451	437	438	7.8	492	513	526	553	572	635
	(28)	(29)	(26)	(27)	(28)		(43)	(45)	(44)	(49)	(50)	(0.009)
YYG	436	459	483	445	447	7.8	506	522	531	556	588	625
	(30)	(35)	(42)	(22)	(29)		(49)	(61)	(60)	(59)	(59)	(0.016)

^[a]

In water at pH 5 or in pure solvents.

^[b]

In water at pH 10 or in solvents containg 50 mM NaOH.

^[c]

In basic (50 mM NaOH) DMF.

^[d]

At pH 13.