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. Author manuscript; available in PMC: 2008 Apr 21.
Published in final edited form as: Anal Chem. 2007 Jan 30;79(5):1897–1906. doi: 10.1021/ac061975q

Figure 1.

Figure 1

Characterization data for films: (a) Background-subtracted, smoothed reversible CV of cyt P450 1B1 on PG electrode at 50 mV/s in pH 7.1 phosphate buffer + 50 mM NaCl. Film composition on electrode was PDDA/DNA/cyt P450 1B1/DNA. (b) Influence of film layers added on QCM frequency decrease for enzymes used in this study. The first three film layers are omitted (RuPVP/DNA/RuPVP) for clarity. (c) Visible difference spectrum for immobilized PDDA/cyt P450cam film on glass microscope slide after reduction to the ferrous form and addition of CO showing the characteristic cyt P450 FeII-CO band near 450 nm.