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. Author manuscript; available in PMC: 2008 Apr 21.
Published in final edited form as: Anal Chem. 2007 Jan 30;79(5):1897–1906. doi: 10.1021/ac061975q

Figure 7.

Figure 7

(a) CapLC chromatogram showing elution of reaction media after 10-min exposure of Mb immobilized in a thin film on SiO2 microspheres to B[a]P/H2O2 (red). Controls are B[a]P only (blue) and 100 mM B[a]P reacted with 0.5 mM H2O2 for 10 min in the absence of Mb (green). (b) MS for the 11–12-min eluting peaks in (a) along with representative epoxides and diol forms of B[a]P. (c) CapLC chromatogram (select ion mode) measuring total ion current m/z 402 from neutral hydrolysate of DNA/Mb film immobilized on carbon cloth after 10-min exposure to 100 mM B[a]P + 0.5 mM H2O2. (d) MS/MS of the 17-min elution peak in (c) with expected fragmentation pattern of parent B[a]P–guanine ion.