Figure 1.—

(A) Chromosomal distribution of the 221 genes that were up- or downregulated in met1-RNAi A. suecica. Colors represent oligonucleotide/gene densities along five chromosomes of A. thaliana from high (red) to low (blue). Blue-colored regions with gaps are locations of low gene densities and coincide with centromeres. Vertical bars represent genes that are up- or downregulated, and the length is proportional to the logarithm-fold changes. The length of chromosomes is shown above chromosome 1. (B) RT–PCR validation of gene expression changes detected by microarrays. The colors of hybridization signals in two biological replicates are shown on the left, and the RT–PCR results are shown on the right. ACT2, action control; control, A. suecica transgenic plants transformed using a GUS reporter; met1-RNAi, A. suecica transgenic plants that overexpress double-stranded MET1.