Figure 8.—

The presence of Muk does not affect the DNA methylation of the distal enhancer and intron 2 regions of P1-wr-mum6. (A) Ethidium bromide-stained gel picture showing PCR-based genotyping of Muk and P1-wr-mum6 individuals. P1-wr [A632] and P1-wr-mum6/ p1-ww [4co63] were crossed with a heterozygous Muk stock to obtain sibling plants with either active or inactive Mu elements. The size of the PCR products is shown on the right. P1-wr-mum6 ears that contain and lack Muk are shown below the gel. (B) DNA gel blot showing the effect of Muk on p1 methylation. A gel blot carrying HpaII-digested DNA of selected genotypes was sequentially hybridized with intron 2-specific probes 8B and 8C and the distal enhancer probe 15. Position and sizes (in kilobase pairs) of bands specific to P1-wr-mum6 are indicated with arrows on the right. Sizes of the molecular weight markers in kilobase pairs are shown on the left.