TABLE 1.
Positions of Mu insertions in P1-wr
| Insertion line | Distance from TSSa | P1-wr region | Mu element(s) types, orientationb | Target sequencec |
|---|---|---|---|---|
| P1-wr-mum1 | −102 | Promoter | Mu1, R | AATTCGGTCGGTCCGTAACGTGC |
| P1-wr-mum2 | −36 | Promoter | Mu1, F | CGTCCGCTGCTATATTATGGCCG |
| P1-wr-mum3 | −34 | Promoter | Mu11, F | TCCGCTGCTATATTATGGCCGGC |
| P1-wr-mum4 | −6 | Promoter | MuDR, ND | CGTGCCCTCTCTAGCCAGCACAG |
| P1-wr-mum5 | +118 | Exon 1 | Mu4, ND | CACCAACTCCCTTGGACGCACGC |
| P1-wr-mum6 | +232 | Exon 1 | Mu1, R | TCCGGTGTGGCCAGCGGCGGCCG |
| P1-wr-mum7 | +412 | Exon 1 | Mu1, F | TGCGGAGCACGGCGAGGGGTCC |
| P1-wr-mum8 | +460 | Intron 1 | MuDR, ND | TAAACCAAAGCCGGCCGCGCGC |
| P1-wr-mum9 | +470 | Intron 1 | Mu1, F; Mu1, F | GCCGGCCGCGCGCCATGCATCGC |
| P1-wr-mum10 | +661 | Exon 2 | Mu1, R | AGGAGGAAGAAGACATCATCATC |
| P1-wr-mum11 | +689 | Exon 2 | Mu1, F | CCACGCCACCCTCGGCAACAGGT |
| P1-wr-mum12 | +701 | Exon 2 | Mu1, R; Mu8, F | CGGCAACAGGTAACAATAAGCGC |
| P1-wr-mum13 | +759 | Inron 2 | MuDR, ND; Mu1, F; Mu1-liked, ND | TAGAGAGTAGTAGTACTACTACT |
a
TSS, transcription start site. The positions of insertions correspond to P1-wr sequence accession EF165349.
b
F, forward orientation; R, reverse orientation; ND, orientation not determinable.
c
The predicted 9-bp target sites of the Mu insertions are indicated in boldface type. Accession no. of P1-wr: EF165349.
d
Mu1-like denotes presence of two SNPs in the TIR sequence that resembles Mu1 (accession no. X00913). SNP positions are underlined in the TIR sequence: 5′-GAATCCCCTTCCCTCTTCGTCCACAATGGCAGTTATC-3′.