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Temperature-dependent morphology of hsp90+, swo1-26, swo1-21, and git10-201 strains. The same strains as shown in Figure 4 were precultured at 28° and then transferred to EMM defined medium and grown for 24 hr at 28°, 30°, and 37°. Cells were heat-fixed and stained with Hoechst 33342 and Calcofluor. Images were visualized and captured using a Zeiss Axioplan2 microscope with an Orca-ER CCD camera and Openlab software.
