Figure 7.—

Analysis of wts, scrib, and roe border cell migration. (A) A stage-9 egg chamber from the wts^x1 clonal analysis experiment without a clone (GFP is expressed in all follicle cells). The follicle cell nuclei are marked with propidium iodide (red), and the actin cortexes with phalloidin (blue). The border cell cluster (arrow) migrates in sync with the trailing edge of the follicular epithelium (TE, arrows). (B) wts^x1 clone in a stage-9 egg chamber (follicle cells without GFP, white dotted outline). The border cell cluster (arrow) remains at the anterior of the egg chamber, while the TE has almost completed migration (arrows). The two nonclone cells marked with GFP at the center of the border cell cluster are the polar cells, which help to organize the cluster and are carried along by the border cells, but do not actively migrate (Szafranski and Goode 2004). (C and D) scrib⁶⁷³ and roe² clones in stage-9 egg chambers. scrib⁶⁷³ border cell clusters (arrow) remain at the anterior of the egg chamber, while the TE has almost completed migration (arrows). roe² has a similar, but less severe phenotype, in which the border cells typically start to migrate when the TE has moved about halfway to the oocyte.