Figure 8.—
Proliferation of Fas2, dlg, lgl, scrib, wts, roe, ft, and mer; ex epithelial cells. (A and B) BrdU incorporation in dlgm52 and wtsX1 clones. dlgm52 cells are marked by absence of β-galactosidase (β-gal) and wtsX1 cells by absence of GFP. PI stains all nuclei, while BrdU labels nuclei that are in or have entered S phase during the 1-hour incubation period. A greater number of BrdU+ cells are observed in dlgm52 and wtsX1 cells compared to nonclone cells. (C) Quantitative analysis of BrdU in Fas2, dlg, lgl, scrib, wts, roe, ft, ex, and mer; ex clones. The bar graph shows the percentage of BrdU+ cells in clones divided by the percentage of BrdU+ cells in nonclones. Control clones were generated with y w P[w+, FRT 101], the parent chromosome for Fas2null and dlgm52. Control clones have a ratio of ∼1, indicating no difference in the percentage of BrdU+ cells in GFP− clone vs. GFP+ nonclone cells. In contrast, the ratio of BrdU+ cells is ∼2-fold greater for Fas2, dlg, lgl, scrib, wts, and roe cells compared to nonclone cells (**P < 0.01; *P < 0.05). ft, ex, and mer; ex cells showed a slight, insignificant decrease in the number of BrdU+ cells. (D and E) PH3 in dlgm52 and wtsX1 clones. dlgm52 cells are marked by absence of β-gal and wtsX1 cells by absence of GFP. PI stains all nuclei, while PH3 labels nuclei that are in mitosis. A greater number of PH3+ cells are observed in dlgm52 and wtsX1 cells compared to nonclone cells. (F) Quantitative analysis of PH3 in Fas2, dlg, lgl, scrib, wts and roe, ft, ex, and mer; ex clones. The bar graph shows the percentage of PH3+ cells in clones divided by the percentage of PH3+ cells in nonclones. Control clones have a ratio of ∼1, indicating no difference in the percentage of PH3+ cells in GFP− clone vs. GFP+ nonclone cells. The ratio of PH3+ cells is ∼2- to 3-fold greater for dlg, lgl, scrib, wts, roe, ft, ex, and mer; ex cells compared to nonclone cells (**P < 0.01; *P < 0.05). Fas2 showed a 10-fold increase in PH3+ cells. Only Fas2, dlg, lgl, scrib, wts, and roe cells showed highly expressive and penetrant PH3 staining after s6, when follicle cells normally stop dividing. (G and H) CycE in dlgm52 and wtsX1 clones. dlgm52 cells are marked by absence of β-gal and wtsX1 cells by absence of GFP. PI stains all nuclei, while CycE labels nuclei that are in mitosis. A greater number of CycE+ cells are observed in dlgm52 and wtsX1 cells compared to nonclone cells. (I) Quantitative analysis of CycE in Fas2, dlg, lgl, scrib, wts, and roe, ft, ex, and mer; ex clones. The bar graph shows the percentage of CycE+ cells in clones divided by the percentage of CycE+ cells in nonclones. Control clones have a ratio of ∼1.2, indicating little difference in the percentage of CycE+ cells in GFP− clone vs. GFP+ nonclone cells. The ratio of CycE+ cells is ∼2- to 3-fold greater for Fas2, dlg, wts, and roe compared to nonclone cells, while lgl and scrib showed a trend toward increased CycE expression. ft, ex, and mer; ex cells showed a significant decrease in CycE expression (**P < 0.01; *P < 0.05).