TABLE 3.
Meiotic progression in 5- to 15-min-old embryos
| Genotype | Embryos scored | Metaphase I (%) | Anaphase I (%) | Metaphase II (%) | Anaphase II (%) | Telophase II (%) | Pronulei (%) | Mitosis (%) | Arrested (%) |
|---|---|---|---|---|---|---|---|---|---|
| wisp41/+ | 128 | 0 (0) | 5 (3.9) | 16 (12.5) | 9 (7.0) | 26 (20.3) | 23 (10.2) | 49 (38.3) | 0 (0) |
| wisp41/Df(1)RA47 | 103 | 1 (1.0) | 5 (4.9) | 11 (12.1) | 4 (3.9) | 16 (15.5) | 16 (15.5) | 0 (0) | 50 (48.5) |
Embryos were collected 10 min after deposition by females of the indicated genotype, fixed for 5 min, and stained with propidium iodide and anti-α-tubulin antibody to score meiotic and mitotic figures. Stages were determined on the basis of spindle and chromosome configurations.