Figure 2.—

Representative Wolbachia density and distribution is shown in embryos at syncytial blastoderm stage, testes and ovaries of wYak-infected, wTei-infected, and wSan-infected D. simulans STCP lines. Wolbachia are stained green-yellow and host nuclei red. Most bacteria are concentrated in the posterior part of the eggs and oocytes. Eggs are oriented with the anterior part to the left. A few bacteria are scattered across the testes, and infected sperm cysts are rare, if present at all, in all three lines. Wolbachia cells are abundant in the ovaries, especially in the early stages of oogenesis for all three lines tested (shown only for wTei-infected ovary). For later stages, no real differences between the three lines are observed. Scale bar: embryos, 100 μm; testes, 100 μm; ovaries, 50 μm.