Figure 4.—

Stability of plasmids evolved in P. putida H2 (pH2) and under DH protocol (pDH), in ancestral and coevolved host H2. •, average stability of the ancestral plasmid in the ancestral host (one assay for each of the five independent ancestral cultures); ▪ and ♦, average stability of the evolved plasmids pH2 in the ancestral host (H2_anc) and in the coevolved host (H2_ev), respectively; ▴, average stability of the plasmids evolved in DH protocol (pDH) in the ancestral host H2_anc. For all evolved plasmids, data represent averages from five evolved plasmids, one from each of the five independent lineages. Error bars represent standard deviations.