Figure 10.—

Alignments of regions of REF4 and its homologs. (A) Region containing the G383 residue substituted in ref4-3. (B) Region containing the D647 residue substituted in ref4-1 and ref4-2. (C) Region containing the P919 residue substituted in the intragenic suppressor mutant. Numbers indicate amino acid positions within the proteins. Arrows indicate substituted residues. Black shading indicates complete conservation; gray shading indicates residues conserved in half or more of the sequences.