TABLE 1.

Analysis of variance and broad-sense mutational heritabilities pooled over sexes

Source	d.f.	MS	F	P	σ2	H2M
24-hr sleep (min)
Sex	1	17,367.650	0.26	0.6078	—	0.45
Line	135	392,439.950	5.46	<0.0001	12,091.70
Line × sex	134	72,022.460	3.66	<0.0001	3,891.80
Error	3597	19,680.100	—	—	19,678.90
Nighttime sleep (min)
Sex	1	2,789,073.880	95.99	<0.0001	—	0.38
Line	135	127,961.060	4.02	<0.0001	3,657.90
Line × sex	134	31,887.210	3.70	<0.0001	1,673.50
Error	3597	8,615.820	—	—	8,615.80
Daytime sleep (min)
Sex	1	2,366,343.690	121.85	<0.0001	—	0.43
Line	135	111,730.850	5.26	<0.0001	3,376.30
Line × sex	134	21,296.810	3.63	<0.0001	1,078.00
Error	3597	5,874.840	—	—	5,874.10
24-hr sleep bout number
Sex	1	2,968.426	22.83	<0.0001	—	0.29
Line	135	301.183	2.11	<0.0001	6.19
Line × sex	134	143.289	4.16	<0.0001	8.19
Error	3597	34.445	—	—	34.48
Nighttime sleep bout number
Sex	1	834.361	21.2	<0.0001	—	0.24
Line	135	127.700	3.00	<0.0001	3.11
Line × sex	134	42.624	2.71	<0.0001	1.93
Error	3597	15.748	—	—	15.77
Daytime sleep bout number
Sex	1	6,944.478	88.49	<0.0001	—	0.33
Line	135	114.519	1.31	0.0580	0.96
Line × sex	134	87.521	6.63	<0.0001	5.46
Error	3597	13.203	—	—	13.20
Average bout length (min)
Sex	1	10,043.693	27.13	<0.0001	—	0.35
Line	135	1,546.851	3.83	<0.0001	43.38
Line × sex	134	404.618	3.31	<0.0001	21.93
Error	3597	122.141	—	—	122.26
Waking activity (counts/min)
Sex	1	2.326	4.01	0.0472	—	0.32
Line	135	1.711	2.69	<0.0001	0.0413
Line × sex	134	0.064	3.85	<0.0001	0.0358
Error	3597	0.166	—	—	0.1655
Glycogen (μg/fly)
Sex	1	1,471.409	14.75	0.0002	—	0.56
Line	135	207.674	1.71	0.0009	38.86
Line × sex	135	121.343	1.91	<0.0001	51.69
Error	256	63.642	—	—	69.99
Triglycerides (μg/fly)
Sex	1	9.381	3.61	0.0589	—	0.60
Line	135	5.849	1.92	<0.0001	0.6104
Line × sex	135	3.059	1.73	<0.0001	2.4210
Error	256	1.758	—	—	1.9920
Protein (μg/fly)
Sex	1	96.850	10.72	0.0012	—	0.56
Line	135	15.802	1.53	0.0070	2.2238
Line × sex	135	10.332	1.65	0.0003	7.8902
Error	257	6.244	—	—	7.9836

MS, mean square. σ², variance component as calculated by restricted maximum-likelihood method. H²_M, broad-sense mutational heritability.