TABLE 2.
esp1 temperature-sensitive mutations
| Codon no. | Expected codon | Found codon | Comment |
|---|---|---|---|
| esp1-N5 | |||
| 1 | MET (ATG) | None (ACG) | Codon 2 is MET |
| 3 | VAL (GTA) | ALA (GCT) | |
| 25 | LEU (TTA) | LEU (CTC) | Silent |
| 63 | ILE (ATA) | LEU (TTA) | |
| 87 | HIS (CAT) | ASN (AAT) | |
| 101 | MET (ATG) | LYS (AAG) | Destroys AflII site |
| 117 | VAL (GTT) | VAL (GTA) | Silent |
| 121 | ASN (AAT) | SER (AGT) | |
| 218 | SER (TCT) | SER (TCC) | Silent |
| 305 | GLN (CAA) | ILE (CTA) | |
| 360 | LEU (TTA) | ILE (ATA) | |
| 479 | ALA (GCT) | ALA (GCC) | Silent |
| 545 | SER (TCA) | THR (ACA) | |
| esp1-N122 | |||
| 90 | ASN (AAT) | SER (AGT) | |
| 511 | CYS (TGC) | PHE (TTC) | |
| esp1-C113 | |||
| 1327 | PHE (TTC) | LEU (CTC) | |
| 1391 | HIS (CAT) | TYR (TAT) | |
| esp1-B3 | |||
| 503 | THR (ACG) | SER (AGC) | |
| 504 | LEU (CTA) | VAL (GTA) | |
| 579 | ASN (AAT) | ASN (GAT) | Silent |
| 603 | ASP (GAC) | ALA (GCC) | |
| esp1-B120 | |||
| 782 | LYS (AAA) | GLU (GAA) | |
| 818 | SER (TCC) | SER (TCT) | Silent |
| 841 | GLY (GGT) | GLY (TCT) | Silent |
| 951 | ILE (ATT) | THR (ACT) | |
| 1040 | ILE (ATA) | THR (ACA) | |