Table 1.
Synthetic oligonucleotides used for isolation and construction of hus3S193 scFv multimer genes
| N4793 | CTG GCG GCC CAG CCG GCC ATG GCC GAG GTC CAA CTG GTG GAG AGC GGT (48 mer) |
| N4810 | GGG ACC CCG GTC ACC GTC TCC TCA GAC ATC CAG ATG ACC CAG AGC CCA AGC AGC CTG (57 mer) |
| N5456 | GTC ACC GTC TCC TCA G (16 mer) |
| N5457 | GA TCC TGA GGA GAC G (15 mer) |
| N5536 | GTC ACC GTC TCC G (13 mer) |
| N5535 | GA TTC GGA GAC G (12 mer) |
| N5911 | GGG ACC CCG GTC ACC GTC TCC GAC ATC CAG ATG ACC CAG AGC (42 mer) |
| N5912 | GCT CTG GGT CAT CTG GAT GTC GGA GAC GGT GAC CGG GGT CCC (42 mer) |
| N5196 | GGG ACC CCG GTC ACC GTC GAC ATC CAG ATG ACC CAG AGC CCA AGC AGC CTG (51 mer) |
| N5183 | CTC GCG GCC CAG CCG GCC ATG GCC GAG ATC CAG ATG ACC CAG AGC CCA AGC AGC CTG (57 mer) |
| N5181 | ACC GCT CTG CAC CAG TTG GAC CTG CCG TTT GAT TTG CTT GGT CCC TTG GCC GAA CGT GAA (63 mer) |
| N5182 | AA CGA GAA TTC TTA CTT GTC ATC GTC GTC CTT GTA ATC TGA GGA GAC GGT GAC CGG GGT CCC TTG GCC CCA GTC (74 mer) |
| N2175 | TGT GTG ATA CGA AAC GA General pPOW3 vector forward primer (17 mer) |
| N2357 | GCG CGT CGG GCT CTA GA General pPOW3 vector reverse primer (17 mer) |
| N4905 | TTT TGT GCA AGA GGC ACC (18 mer) |
| N4796 | TGT GAT TTG CAG CTT GGT CCC TTG GCC GAA (30 mer) |
| N5205 | GCT TGG GCT CTG GGT CAT CTG G (22 mer) |
Nucleotide sequence of the oligonucleotide primers used to attach the linkers between the VR and the VL domains to form the hu3S193 scFv dimer, trimer, and tetramer molecules.