Figure 3.

Effects of the mutations in PfuPCNA on the DNA synthesis reaction by P. furiosus Pol I. The reaction mixtures with indicated PfuPCNA proteins in each lane, both in the presence and absence of PfuRFC, were analyzed by 1% alkali agarose gel electrophoresis, and the products were visualized by autoradiography. The sizes indicated on the left were from BstI-digested λ phage DNA labeled by ³²P at each 5′ end.