Table 2.

Charge and α-helix content of rhGCSF under different pH conditions

	Chargea
pH	+	−	Net	% α-Helix contentb
3.5	14	0	14	84% at pH 2.5
6.1	13	13	0	75% at pH 4.5
7.0	9	13	−4	66% at pH 7.5

^a Charges were calculated based on rhGCSF amino acid composition found in Protein Data Bank. pKa values of charged residues (Asp, Glu, Lys, Arg, and His) were obtained from Lehninger, (Lehninger et al. 1993).

^b % α-Helix contents were taken from Narhi (Narhi et al. 1991).