Table 1.

Pantothenate synthetase activity assays (sec−1)

	Full lengtha	Truncatedb
Wild type	1.74 ± 0.12	1.66 ± 0.15
E77G	1.52 ± 0.02	1.58 ± 0.34
Butyratec
10 mM	1.41
50 mM	1.11
100 mM	0.973
Glycerolc
274 mM	1.51
685 mM	1.51

^a Full-length protein is the protein before enterokinase digestion, with the N-terminal fusion tag.

^b Truncated protein is the protein after enterokinase digestion.

^c The effects of Na butyrate and glycerol on the activity are measured with the full-length E77G mutant protein. These are single measurements.