Table 1.

Characterization of MTGAL, HIGAL, and AAGAL

Enzyme	MTGAL	HIGAL	AAGAL
pH optimum (at 37°C)	7.0	8.5	4.0
pH of crystallization	8.5	6.5	7.5
pH range where activity > 50%	5.5–8.5	6.5–9.5	3.0–5.5
Temperature optimum	65°C (at pH 6.5)	65°C (at pH 6.5)	37°C (at pH 6.5)
			50°C (at pH 3.5)
pH stability rangea	3.5–8.5	3.5–8.5	2.5–5.5
Theoretical pIb	6.0	6.7	4.0

^a This is the pH interval where activity > 50% is left after incubation for 2 h at 37°C.

^b Calculated in http://us.expasy.org/tools/protparam.html.