Table 2.

HX parameters of RNase A and S, and Cyt c with or without 1 M sucrose

		HX rate constantsa (h−1)
Proteins	HX rates	0 M sucrose	1 M sucrose	HX retardation factorb	ΔΔGHXc (kcal/mole)
RNase A	Fast	0.70	0.63	1.11	0.06
	Slow	0.0025	0.0013	1.92	0.39
RNase S	Fast	0.64	0.53	1.21	0.11
	Slow	0.0047	0.0038	1.24	0.13
Cyt c	Fast	2.13	0.60	3.55	0.75
	Slow	0.00036	0.00034	1.06	0.35

^a Values were obtained by fitting with equation 3 for the triplicate samples for RNase A and S and duplicate samples for Cyt c.

^b Calculated by k₀/k_s, where k₀ and k_s are HX rate constant with and without 1 M sucrose, respectively.

^c Calculated by the ΔΔG_HX = −RT ln(k₀/k_s).