Table 1.
Comparison of 15N relaxation parameters, residual dipolar couplings, and Stokes radius for Δ131Δ without versus with a denaturing perturbation (left column)
| r (NOE) | r (relaxation interference) | r (R2) | r (RDC) | rg/rgΔ131Δ,pH 5.2 | |
|---|---|---|---|---|---|
| Acid (pH 3.0) | 0.98 | 0.92 | 0.94 | 0.98a | n.a. |
| Urea (8 M) | 0.80 | 0.81 | 0.53 | 0.82b | 1.17b |
| Sextuple | 0.98 | 0.88 | 0.74 | 0.94b | 1.05b |
| Sextuple + urea | 0.78 | 0.74 | 0.33 | 0.79b | 1.18b |
| Oxo-sextuple | 0.73 | 0.83 | 0.53 | 0.92b | 1.08b |
a M.S. Ackerman and D. Shortle, unpubl.
b Data are taken from Ackerman and Shortle (2002b)).
r is the Pearson correlation coefficient between the two data sets. rg is the Stokes radius.