Table 3.
Distancea |
|||||
---|---|---|---|---|---|
Ligand | Water | Proteinb | Human | Escherichia coli | Yeast |
PLPc | |||||
N1 | W1 | Trp206A NE1 | 3.3:3.2 | 3.4:3.3 | |
O3′ | His227B NE2 | 2.7 | 2.6 | ||
O4′ | W2 | Glu77A OE1 | 3.1:2.6 | 3.1:3.5 (Asp) | |
OP1 | Lys100A NZ | 2.7 | 3.0 | ||
OP2 | Tyr157A OH | 2.8 | 2.5 | ||
OP2 | Arg161A NE | 2.7 | 2.6 | ||
OP3 | Arg161A NH1 | 2.9 | 2.7 | ||
OP3 | Arg225B NH2 | 2.7 | 2.9 | ||
OP3 | Ser165A OG | 3.5 | — | ||
OP3 | W3 | Ser165A OG | — | 2.7:2.5 | |
FMN | |||||
O2 | Thr111A OG1 | 2.8 | 2.8 | 3.1 (Ser) | |
O2 | Glu174A NE2 | 3.1 | 3.1 | 3.1 | |
N3 | Phe110A O | 2.9 | 2.8 (Tyr) | 3.6 (Tyr) | |
O4 | Leu98A N | 3.3 | 3.2 | — | |
O4 | W4 | Phe110A N | 2.7:2.7 | 2.7:2.8 (Tyr) | — |
N5 | Leu98A N | 3.4 | 3.4 | — | |
O2* | Met96A O | 2.7 | 2.7 (Ile) | 2.3 (Ile) | |
O2* | Gln139B NE2 | 3.1 | 2.8 | 3.2 | |
O3* | Trp219B NE1 | 3.4 | 3.5 | 3.5 | |
O3* | W5 | Pro261B OXT | 2.7:3.0 | 2.8:2.8 | — |
O3* | W6 | Trp219B NE1 | 2.8:2.5 | 2.8:2.8 | — |
O3* | W7 | Glu217B OE1 | 2.7:2.5 | 2.7:3.1 | |
O4* | Lys117A NZ | 2.7 | 2.8 | — | |
O4* | Gln174A NE2 | 3.1 | 3.2 | — | |
O4* | Arg138A NH2 | — | — | 2.7 | |
O4* | Arg229A NH2 | — | — | 2.9 | |
O5* | Lys117A NZ | 3.4 | 3.3 | 2.7 | |
O5* | Arg141B NH1 | 3.3 | — | — | |
O1P | Arg95A NE | 2.8 | 2.7 | 2.6 | |
O1P | Arg141B NH2 | 2.8 | — (Met) | 2.9 | |
O1P | W8 | Arg116A NH1 | — | 2.9:2.5 | — |
O2P | Ser175A OG | 3.5 | 3.1 | 2.4 | |
O2P | Arg116A N | 3.2 | 3.3 | — | |
O2P | Lys117A NZ | 2.9 | 2.8 | — | |
O3P | Arg229B NH2 | 2.8 | 3.0 | 2.9 | |
O3P | Ser175A OG | 2.7 | 2.6 | — | |
O3P | Arg116A NE | — | 3.1 | 2.9 | |
O3P | Lys117A N | — | — | 2.6 | |
O3P | W9 | Arg116A NH2 | 2.9:2.9 | — | — |
a Interactions that are less than 3.6 Å are listed.
b Nonidentical residues are shown in parentheses.
c Only the human and E. coli PNPOx have PLP bound at the active site.