Figure 1.

Sequences of peptides used in this study. Peptides are denoted by one-letter code and are labeled peptides 1 to 7. The sequence of GCN4-p1 (O’Shea et al. 1991) residues 2–32 is shown for comparison, where Met 2 of GCN4-p1 is labeled residue 1. Hybrid 1 is a GCN4-cortexillin I hybrid that did not fold into a coiled-coil, whereas Hybrid 2 formed a stable coiled-coil (Lee et al. 2001). The a and d positions of the heptad repeat abcdefg are boxed. Bolded residues indicate amino acid substitutions in Hybrid 2 relative to Hybrid 1. The circled and bolded residues in peptides 2 to 7 indicate amino acid substitutions relative to the Hybrid 2 peptide sequence. The residues 2–14 in the Hybrid 1 sequence are from cortexillin I residues 270–282, and residues 16–28 contain GCN4-p1 2–14, where both segments are inserted into the GCN4 template 1–31.