Table 1.

Flap residues that undergo slow dynamics on msec-μsec time-scale in protease ligand complexes detected using 15N relaxation data

PR	Inhibitor	KDa	Residue numbers
PRb	DMP325c	10−9 M	50, 51	50, 51
PRb	P9941c	10−8 M	50, 51	50, 51
PRb	KNI272d	10−11 M	48–51	148–153
PRD25N	substrate C	10−4 M	46–48, 50, 51, 54e	149, 151e

^a Ligand dissociation constant.

^b The PR used in these studies did not contain the Q7K/L33I/L63I mutations.

^c (Nicholson et al. 1995).

^d (Freedberg et al. 1998).

^e Relaxation data were not measured for residues 50, 51, and 151 because of severe signal broadening, presumably due to conformational exchange on the msec-μsec time scale.