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Hydrodynamic properties of IF7. (A) Determination of the Stokes radius by gel filtration chromatography on an HR Superdex G75. The elution volume of IF7 is indicated by an arrow and a square (see text for details). The numeration corresponds respectively to the elution volumes of ribonuclease A (1), chymotrypsinogen A (2), ovalbumin (3), and albumin (4). (B) Gel filtration elution peak of IF7 in phosphate buffer, pH 6.5 (10 mM) at 298 K; flow, 1mL/min.
