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Zinc content of the wild type and mutant enzymes
| Zinc/enzyme ratio |
|||
|---|---|---|---|
| Added Zn(II) during dialysis (μM) | Wild type | R121H | R121E |
| 0 | 1.4 | 1.5 | 1.2 |
| 5 | 1.5 | 1.8 | 1.9 |
Protein samples (1 mL 60–80 μM) were dialyzed against 150 mL of a buffer containing 15 mM Hepes at pH 7.5 (1) treated with chelex and (2) with 5 μM added ZnSO4. Five buffer changes were performed. The values correspond to the average of two independent determinations. Standard deviations were below 10%.
