Table 1.
Stern-Volmer constants obtained for dynamic and static modes of quenching by acrylamide via steady-state measurements
| Without CaM |
With CaM |
|||
|---|---|---|---|---|
| Sample | KSV (M−1) | V (M−1) | KSV (M−1) | V (M−1) |
| NATA | 17.9 ± 0.6 | 1.5 ± 0.1 | — | — |
| hMBP-Cit0 | 9.60 ± 0.20 | 1.19 ± 0.04 | 4.73 ± 0.21 | 0.53 ± 0.08 |
| hMBP-Cit6 | 8.44 ± 0.27 | 1.35 ± 0.06 | 4.81 ± 0.39 | 1.28 ± 0.14 |
| rmMBP-Cit0 | 9.31 ± 0.33 | 1.58 ± 0.07 | 4.14 ± 0.12 | 0.88 ± 0.05 |
| rmMBP-qCit6 | 9.11 ± 0.26 | 1.49 ± 0.06 | 4.54 ± 0.18 | 1.30 ± 0.06 |
All experiments were performed in 50 mM TRIS-HCl (pH 7.4) 250 mM NaCl, and 1 mM CaCl2. The MBP and CaM concentrations were 2 μM. The data represent averages of three experiments. The regression coefficients for fits to Equation 5 in the text were all r2 > 0.99.