Table 1.
Residue | Codon | Replacement | Codon |
Ala | GCN | Gly | GGN |
Asp | GAY | Asn | AAY |
Glu | GAR | Gln | CAR |
Phe | TTY | Tyr | TAY |
Gly | GGN | Ala | GCN |
His | CAY | Asn | AAY |
Ile | ATH | Leu | CTH |
Lys | AAR | Arg | AGR |
Leu | CTH | Ile | ATH |
TTR | ATR | ||
Met | ATG | Leu | CTG |
Asn | AAY | Asp | GAY |
Pro | CCN | Ala | GCN |
Gln | CAR | Glu | GAR |
Arg | AGR | Lys | AAR |
Ser | TCN | Thr | ACN |
AGY | ACY | ||
Thr | ACN | Ser | TCN |
Val | GTN | Leu | CTN |
Trp | TGG | Leu | TTG |
Tyr | TAY | Phe | TTY |
Amino acids were mutated to the closest homologs that can be reached with only one base change. Codons used are shown above, with relevant degeneracies indicated with IUPAC nomenclature (N = A/C/G/T; Y = C/T; R = A/G; H = A/C/T). Changed base is indicated in bold. Cysteine is not shown, as there are no cysteines in the P99 enzyme.