Figure 3.

Sequences, calculated disruption, and effective level of mutation of functional lactamases. Closed triangles indicate profile minima, and filled and open blocks represent TEM-1 and PSE-4 sequences, respectively. The calculated disruption E represents the number of interactions broken by recombination. Minimal inhibitory concentrations of ampicillin (MIC) are in μg/mL and were determined using liquid cultures. Effective level of mutation (m) is the minimum number of mutations required to convert a chimera into one of its parents at only those residues recognized by SCHEMA, that is, residues whose coordinates are defined in the TEM-1 structure (Jelsch et al. 1993).