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Electrostatic free energy curves for the membrane interaction of the δ1 and δ3 PH domains. The electrostatic free energy of interaction (in kcal/mole) with 2 : 1 PC/PS (A) and 5 : 1 PC/PS (B) membranes in 0.1 M KCl as a function of the distance between the van der Waals surfaces of the PH domain and membrane. The electrostatic free energies were calculated using the finite difference Poisson-Boltzmann (FDPB) method as described in Materials and Methods (Gallagher and Sharp 1998).
