Figure 9.

The effect of phosphorylation of Ser26 on the electrostatic properties of PLCβ3-PH. The PH domain is oriented differently than in Figure 7 ▶ so that the surface containing Ser26 is facing the viewer. (A, B) Electrostatic equipotential contours are shown for PLCβ3-PH in the unphosphorylated (A) and phosphorylated (B) states. The Cα backbone is colored green at the position of Ser26. The blue and red meshes represent, respectively, the +1 kT/e and −1 kT/e equipotential contours. (C, D) The electrostatic potentials are mapped to the molecular surface of PLCβ3-PH in the unphosphorylated (C) and phosphorylated (D) states. The surface potentials are color-graded from −4 kT/e (red) to +4 kT/e (blue). All potentials were calculated with 0.1 M KCl and visualized in GRASP (Nicholls et al. 1991).