. 2003 Sep;12(9):1934–1953. doi: 10.1110/ps.0358803

Table 1.

Comparison of the electrostatic free energy of interaction of the PLCδ1 PH domain in the absence and presence of bound Ins(1,4,5)P3 with a 2 : 1 PC/PS membrane in 0.1 M KCl

R^a (Å)	δG_el (kcal/mole), δ1-PH, no Ins(1,4,5)P₃	δG_el (kcal/mole), δ1-PH with bound Ins(1,4,5)P₃
0	0.36	11.3
0.5	−1.3	8.7
1.0	−2.3	6.3
1.5	−3.6	4.0
2.0	−3.9	2.7

^a R is the distance between the van der Waals surfaces of the PH domain and membrane. The electrostatic free energies were calculated by using the finite difference Poisson-Boltzmann (FDPB) method as described in Materials and Methods (Gallagher and Sharp 1998).