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Heat capacity plot of observed ΔH values for binding PEP to EI(H189A) in the presence of 2 mM Mg2 and Buffer A (pH 7.5) versus temperature (15, 20, and 25°C) from ITC measurements. The slope (ΔCp) is shown (−0.33 ± 0.03 kcal K−1mole−1) with errors for each titration indicated.
