. 2003 Sep;12(9):2087–2098. doi: 10.1110/ps.03192503

Table 2.

S. chromofuscus rPLD activity toward phosphatidylcholine substrates

Substrate	Concentration (mM)	Physical state	Specific activity^a (μmole min⁻¹ mg⁻¹)
diC₄PC	5	Monomer	14^b
diC₆PC	5	Monomer	41^b
diC₇PC	0.5	Monomer	54^b
	5	Micelle	68^b
1-C₆PC	5	Monomer	33^b
POPC	10	SUVs	11.7^c
	10	LUVs	12.5^c
POPE	10	SUVs	3.7^c
	10	LUVs	1.5^c
DOPMe	10	SUVs	0.44^c
	10	LUVs	0.80^c
POPG	10	SUVs	0.013^c
POPC/DOPMe	5/5	SUVs	11.9 (PC)/0.46 (PMe)^c
POPC/DOPMe/chol	5/5/5	SUVs	11.5 (PC)/0.93 (PMe)^c
POPS	10	SUVs	0.0020^c
PI	5	SUVs	0.058^c
SM	5	SUVs	0.0021^c

^a Errors in specific activities (rates for given assay samples were done in duplicate) are <15%.

^b Activities determined by pH-stat at pH 8.0 with 5 mM Ca²⁺ present.

^c Activities determined by ³¹P NMR at pH 7.0 in 20 mM imidazole and 5 mM Ca²⁺ (except for PI, which had 1 mM Ca²⁺).