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. 2008 Mar 7;8:44. doi: 10.1186/1471-2180-8-44

Table 1.

Primers used in this study.

Primer Purpose Sequence (5' to 3') a,b Product size (bp)
repA-UNI-5 universal repA PCR GGA TGT GGT GAG TGC CAG TTC A 898
repA-UNI-3 universal repA PCR CCG CTG YTC GGT CAT CTG C
repA-SEQ-5 repA sequencing species-specific PCR CAG CAG GCC GAC GAC TCG 623
repA-SEQ-3 repA sequencing ATC GGC TGC TTG CGY TCG GT
repA-636-3 species-specific PCR GTC GAG CGC GAG CAT CGG C 636
repA-237-3 species-specific PCR CCA CAC GCG GCG GGT GGT 237
parB-UNI-5 universal parB PCR
parB sequencing
GCA GCA GGC GAT YCA CGT G 656
parB-UNI-3 universal parB PCR
parB sequencing
CGT CGC GCT TST CCT TCG G

a Standard code, Y = C or T; S = C or G

b Mis-matched nucleotide positions in the species specific primers are underlined