Table 1.
Primer | Purpose | Sequence (5' to 3') a,b | Product size (bp) |
repA-UNI-5 | universal repA PCR | GGA TGT GGT GAG TGC CAG TTC A | 898 |
repA-UNI-3 | universal repA PCR | CCG CTG YTC GGT CAT CTG C | |
repA-SEQ-5 | repA sequencing species-specific PCR | CAG CAG GCC GAC GAC TCG | 623 |
repA-SEQ-3 | repA sequencing | ATC GGC TGC TTG CGY TCG GT | |
repA-636-3 | species-specific PCR | GTC GAG CGC GAG CAT CGG C | 636 |
repA-237-3 | species-specific PCR | CCA CAC GCG GCG GGT GGT | 237 |
parB-UNI-5 | universal parB PCR parB sequencing |
GCA GCA GGC GAT YCA CGT G | 656 |
parB-UNI-3 | universal parB PCR parB sequencing |
CGT CGC GCT TST CCT TCG G |
a Standard code, Y = C or T; S = C or G
b Mis-matched nucleotide positions in the species specific primers are underlined