Figure 6.

Cellular localization of EhRAD51 in non-irradiated (No UV-C) and irradiated (UV-C) trophozoites at 30 min, 3 h and 12 h after treatment. Trophozoites were incubated with anti-rEhRAD51 antibodies, treated with FITC-labeled secondary antibodies, counterstained with propidium iodide (PI) and analyzed through confocal immunofluorescence microscopy. A-P. EhRAD51 immunodetection. A, E, I and M, green channel (FITC); B, F, J and N, red channel (PI) channel; C, G, K and O, merge images; D, H, L and P, single cell (100× magnification) from boxes denoted in C, G, K and O.Q. Occurrence of EhRAD51 nuclear foci. The percentage of trophozoites displaying EhRAD51 foci was calculated after scoring 50 nuclei for each time point. Data are the mean of three independent assays.