Figure 7.

Immunohistochemical analysis of the proteins for the regulatory subunits of PP2A-A and PP2A-B in the retina, lens epithelium, lens fiber and cornea of 15.5-day-old mouse eye. The experimental procedures are described in the Methods section. The two antibodies used for western blot analysis in Figure 6 were also used here in immunohistochemical analysis. Note that both A and B subunits of PP-2A were strongly expressed in the cornea. In the retina, the B subunit is homogenously expressed in both the neuro-retina layer and pigment retina layer. In contrast, the expression of the regulatory A subunits is stronger in the pigment layer than in the neuro-retina layer. Expression of both A and B subunits of PP-2A seems stronger in the fiber cells than in the lens epithelium. In addition, while the A subunit appears in both the central and cortical fibers, B subunit is mostly distributed in the cortical fibers. A: hematoxylin and eosin (HE) staining of the section cut from15.5-day-old mouse eye. B: The section cut from the same embryonic mouse eye of A was used for immunohistochemistry analysis with normal serum. C: The section from the same embryonic mouse eye of A was used for immunohistochemistry staining with anti-PP2A-Aα/β antibody. Note that PP2A-Aα/β expression was different in the two layers of retina. D: The section from the same embryonic mouse eye of A was used for immunohistochemistry staining with anti-PP2A-B α/β/γ antibody. Note that PP2A-Bα/β/γ expression was homogenous in both layers of retina.. Bar: 0.2 mm.