Figure 3.

Immunohistochemical analysis for the effect of LPS on vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) expression. Cells were visualized with rhodamine-conjugated Con A (Con A, in red). Reaction products with antibodies for VCAM-1 and ICAM-1 were visualized with an AF488-conjugated second antibody (AF488, in green) in human neonatal dermal LEC. Merged images indicate that expression of VCAM-1 was not observed in LEC but was observed in the cells with LPS, and that expression of ICAM-1 was observed in LEC and strongly in the cells with LPS. Bar = 100 μm.